Next Generation Sequencing
Whole Exome Sequencing
Whole Exome Sequencing (WES) focuses on sequencing the protein-coding regions of the genome (the exome), which comprise about 1-2% of the genome but contain approximately 85% of known disease-related variants.
WES at TATAA
Whole Exome Sequencing, WES, is often used in medical research and diagnostics to identify genetic mutations associated with diseases, particularly in cases where whole genome sequencing (WGS) may be unnecessary. It is a powerful tool for discovering genetic causes of inherited disorders, cancer, and other complex diseases.
- Similar applications to WGS, but focuses on exonic regions only.
- Does not capture structural variants, non-coding regions, regulatory elements, or copy number variants.
- More cost-effective and data-efficient compared to WGS.
PCR ASSAYS AT TATAA
TATAA Biocenter specializes in developing and validating quantitative nucleic acid analysis (qPCR and dPCR) for bioanalysis, adhering to regulatory requirements for different phases. Our services encompass fit-for-purpose assays to full Good Laboratory Practice (GLP) and Good Clinical Laboratory Practice (GCLP) compliant assays. We ensure GLP accreditation for preclinical and GCLP compliance for clinical samples, meeting FDA and EMA standards.
Our core expertise is quantifying low-copy RNA and DNA in intricate biological samples. Our services encompass consultation on sampling, storage, transportation, experimental planning, instrument selection, and reagent recommendations. We excel in extractions from diverse tissue types, biofluids, and various sampling tools.
WES
Library preparation
IDT xGen DNA Library Prep Kit EZ with UDIs, combined with xGen Exome Hyb Panel v2
Recommended sample input
- Fresh frozen cells or tissue
- EDTA whole blood or PAXgene DNA tubes
- High quality FFPE (IDT DNA Integrity score > 1.0)
Recommended DNA input
Range: 0.1 ng – 1 ug
Standard input: 100ng
Recommended sequencing
Paired-end sequencing
Deliverables
Quality-controlled data
Fastq files
Study report
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