Publications

The MIQE Guidelines

ESTIMATED READING TIME: 2 minutes

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Abstract (from Clinical Chemistry)

Background: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader’s ability to evaluate critically the quality of the results presented or to repeat the experiments.Content: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.

Summary: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results


Read More

Archive

Biomarker testing in drug development
T-cell cytotoxicity assays
Viral shedding
Recommendations and Analysis of Shedding Studies by the FDA
PCR for short interfering RNA (siRNA) and ASOs
Biodistribution for cell and gene therapy

Share

  • dPCR, MIQE, PCR
SERVICES
about TATAA
TECHNOLOGIEs
PRODUCTS
CONTACT
Group 3 Terms and Conditions
Group 3 Privacy Policy
Sign up to our newsletter

"*" indicates required fields

Name*
Linkedin-in

TATAA Biocenter AB © 2024 All rights reserved​​

×

Olink

"*" indicates required fields

What platform of interest?*
Name*
Consent*
Consent

General Enquiries

Please fill in the form below and we’ll get back to you ASAP.

"*" indicates required fields

Name*
Consent*
Consent

Courses

Please fill in the form for any courses request

"*" indicates required fields

Name*
Consent*
Consent

Sign up to our Newsletter

"*" indicates required fields

Name*

Bioanalytical Services

Please fill in the form below and one of our bioanalytical experts will get back to you ASAP.

"*" indicates required fields

What services are you interested in?*
Hidden
Untitled
Hidden
Consent*
Consent

Products

Please fill in the form below for any products request

"*" indicates required fields

Consent*
Consent