A patent for the Two-Tailed (2T) PCR technology invented by TATAA Biocenter has been granted in the United States:
Two-Tailed (2T) PCR has an important advantage when the target nucleic acid is short. An animation showing the mechanism is available here.
First assays reaching the market are R for microRNA analysis, available through Biovendor. In development are methods and assays for DNA analysis using digital PCR, allowing rare sequence variants to be detected with unprecedented sensitivity and specific in material of compromised quality.
Two-Tailed PCR also has important advantages for the characterization of siRNAs, Antisense Oligonucleotides (ASOs) and CRISPR constructs developed for Cell and Gene Therapy applications .
Notes on the patent (Abstract from United States Patent)
Methods and compositions for nucleic acid detection
The present invention provides methods, compositions, reaction mixtures, kits, and/or systems for producing a complementary sequence to a region in a target polynucleotide in a sample. These methods, compositions, reaction mixtures, kits, and/or systems are particularly useful for detecting short nucleic acids.
In some aspects, the methods, compositions, reaction mixtures, kits, and/or systems comprise subjecting the sample to a nucleic acid amplification reaction in a reaction mixture under conditions to yield the complete sequence to the region of the target polynucleotide. In some respects, the complementary sequence produced is amplified. You can read more about the patent here.
