Nucleic acid extraction
Extraction
An optimized extraction process ensures reduced variability and improved data accuracy.
With controls and spike-ins in place for complete transparency throughout the extraction process, we provide visibility into yield and nucleic acid integrity, ensuring that extractions can be reproducibly compared across different sample types and test items.
DNA/RNA extraction
The extraction process aims to maximize the recovery of the test item, ensuring the capture of nearly all copies with the highest possible nucleic acid integrity. Eliminating any substances that could interfere with downstream reactions is also crucial. When comparing multiple test items across samples, it’s essential to maintain balanced recovery, ensuring no item is favored over another.
The sample type
Extraction methods vary depending on the biological sample type, and each needs to be optimized for best results. We have developed and optimized, often automated, workflows for various sample types to ensure high-quality nucleic acid extractions.
- Blood and cultured cells are simple matrices that require care to avoid inhibitors and contaminants.
- Fibrous or fatty tissues: Require specific protocols for efficient lysis due to their tough or lipid-rich nature.
- Complex excreta samples (e.g., feces and urine): High in polysaccharides, salts, and bacteria, making it challenging to isolate clean nucleic acids without contamination.
- Formalin-Fixed, Paraffin-Embedded (FFPE) tissues: While preserving tissue morphology, FFPE samples can degrade DNA and RNA, making it challenging to extract high-quality nucleotides.
- Encapsulated viral DNA: Requires specialized protocols to break open viral particles and release the nucleic acids.
PCR ASSAYS AT TATAA
TATAA Biocenter specializes in developing and validating quantitative nucleic acid analysis (qPCR and dPCR) for bioanalysis, adhering to regulatory requirements for different phases. Our services encompass fit-for-purpose assays to full Good Laboratory Practice (GLP) and Good Clinical Laboratory Practice (GCLP) compliant assays. We ensure GLP accreditation for preclinical and GCLP compliance for clinical samples, meeting FDA and EMA standards.
Our core expertise is quantifying low-copy RNA and DNA in intricate biological samples. Our services encompass consultation on sampling, storage, transportation, experimental planning, instrument selection, and reagent recommendations. We excel in extractions from diverse tissue types, biofluids, and various sampling tools.
The Test item
Nucleic acids are not all extracted equally. Even the same molecule type, such as siRNA, can have variable recovery rates depending on factors like modifications, secondary structure, length, and other chemical properties. These variations are expected as long as the extraction process is robust, reproducible, and transparent.
- DNA: Varies in extraction efficiency due to different sample types, requiring specific protocols to ensure high yield and purity.
- cfDNA: Often present in low amounts, cfDNA extraction requires sensitive methods to capture these small, fragmented molecules from biofluids.
- Total RNA: Total RNA extraction includes coding and non-coding RNAs, with variations in yield based on the tissue or sample type.
- miRNA: MicroRNAs are small and challenging to extract due to their size and structure, often requiring specialized protocols.
- mRNA: mRNA enrichment is generally straightforward but can be impacted by the sample’s RNA integrity and poly-A tail length.
- Drug test items (AAV vectors, siRNA, etc.): Different chemical modifications and secondary structures of therapeutic molecules can influence extraction efficiency, requiring optimized protocols for each type.
Stay in touch
Sign up for our newsletter
test
"*" indicates required fields
Automated workflows
Enhance precision and productivity with automation
Most pipetting steps can be fully automated with our advanced liquid handling platforms.
Automation standardizes processes, ensuring consistent and precise results across experiments. By automating repetitive tasks such as sample handling and assay preparation, we can manage a higher volume of samples, accelerate project timelines, and easily scale to accommodate studies ranging from preclinical to large clinical cohorts. Our automated systems are fully integrated with our LIMS, ensuring accurate sample tracking, data traceability, and regulatory compliance.
Services
Solutions for complex biological challenges
We offer a broad range of tailored commissioned services for drug development programs. With over two decades of expertise in nucleic acid analysis and sample preparation, we optimize workflows by assessing sample quality, validating performance, and analyzing clinical biomarkers using leading, well-matched technologies.
About us
The power of our approach
Committed to quality, innovation, and fostering strong client relationships, we serve as your trusted partner to accelerate drug development. As a contract research organization, our mission is to push the boundaries of science and technology to generate accurate and reproducible data that shortens time-to-market.
Regulated laboratory environment
We are GLP accredited for qPCR, dPCR, and molecular biology, GCLP compliant, and ISO/IEC 17025 accredited. Our facility features a strict sample management process, a fully integrated LIMS system, backup for all vital systems, temperature and humidity control, and robust IT security.
Purpose-built PCR laboratory
Our purpose-built laboratory in Gothenburg, Sweden, is specifically designed for PCR. It has controlled air pressure, temporal separation, and biosafety cabinets to minimize contamination risk. This setup enables us to achieve the highest sensitivity and robustness required for validated assays. The lab is equipped with market-leading PCR and NGS instruments and advanced liquid handling systems.
Pioneers in assay development and validation
We are a team of 45 employees, with scientists at the forefront of assay development, optimization, and validation. Our team has co-authored key publications in the field, including Recommendations for Method Development and Validation of qPCR and dPCR Assays in Support of Cell and Gene Therapy Drug Development (AAPS J. 2024) and The MIQE Guidelines for qPCR and dPCR.
Flexible, client-centered solutions
We work closely with our clients to find tailored solutions, offering flexibility in sample types, test items, sample volume, and scalability. We prioritize transparency and proactive communication throughout each project, ensuring our clients are continuously updated on the progress.
contact
Let's explore how we can support your drug development program.
One form for all pages, including link to mailchimp.
"*" indicates required fields
