Next Generation Sequencing
Small RNA-Seq
Sequencing targets small RNA species (typically 18-30 nucleotides), such as microRNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs), capturing RNA-mediated gene regulation.
miRNA and siRNA sequencing
Small RNA-Seq enriches small RNA molecules, providing quantitative insights into how they regulate gene expression at the post-transcriptional level. This method can be used to evaluate the potential of small RNAs as therapeutic targets or to assess the efficacy of drugs that modulate gene expression.
Identify miRNAs and siRNAs involved in disease pathways.
Study RNA interference (RNAi) mechanisms when developing RNA-targeted therapies.
miRNA biomarker discovery and validation in early drug development stages.
PCR ASSAYS AT TATAA
TATAA Biocenter specializes in developing and validating quantitative nucleic acid analysis (qPCR and dPCR) for bioanalysis, adhering to regulatory requirements for different phases. Our services encompass fit-for-purpose assays to full Good Laboratory Practice (GLP) and Good Clinical Laboratory Practice (GCLP) compliant assays. We ensure GLP accreditation for preclinical and GCLP compliance for clinical samples, meeting FDA and EMA standards.
Our core expertise is quantifying low-copy RNA and DNA in intricate biological samples. Our services encompass consultation on sampling, storage, transportation, experimental planning, instrument selection, and reagent recommendations. We excel in extractions from diverse tissue types, biofluids, and various sampling tools.
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Small RNA-Seq
Library preparation
Takara
Recommended sample input
- Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized)
- Tissue or cells (fresh-frozen or stabilized)
- Plasma, serum or other biofluids
Recommended DNA input
Range: 1 ng – 1 ug
Standard input: 10ng
RIN > 8
Recommended sequencing
Single-read sequencing
Deliverables
Quality-controlled data
Fastq files
Study report
Additional options
Differential gene expression analysis, pathway analysis, custom data analysis (please enquire to discuss further).
TWO-Tailed PCR technology
Short RNA quantification with qPCR/dPCR
A TATAA Biocenter innovation that offers validated qPCR/dPCR workflows for siRNA, miRNA, ASOs, and other short nucleic acid targets.
PCR bioanalysis offers several advantages over other methods in speed and throughput; however, standard PCR is unsuitable for short targets due to its length. We have developed a two-tailed primer that enables the optimization, validation, and execution of PCR-based bioanalysis on any short target with high sensitivity and specificity.
About us
The power of our approach
Committed to quality, innovation, and fostering strong client relationships, we serve as your trusted partner to accelerate drug development. As a contract research organization, our mission is to push the boundaries of science and technology to generate accurate and reproducible data that shortens time-to-market.
Regulated laboratory environment
We are GLP accredited for qPCR, dPCR, and molecular biology, GCLP compliant, and ISO/IEC 17025 accredited. Our facility features a strict sample management process, a fully integrated LIMS system, backup for all vital systems, temperature and humidity control, and robust IT security.
Purpose-built PCR laboratory
Our purpose-built laboratory in Gothenburg, Sweden, is specifically designed for PCR. It has controlled air pressure, temporal separation, and biosafety cabinets to minimize contamination risk. This setup enables us to achieve the highest sensitivity and robustness required for validated assays. The lab is equipped with market-leading PCR and NGS instruments and advanced liquid handling systems.
Pioneers in assay development and validation
We are a team of 45 employees, with scientists at the forefront of assay development, optimization, and validation. Our team has co-authored key publications in the field, including Recommendations for Method Development and Validation of qPCR and dPCR Assays in Support of Cell and Gene Therapy Drug Development (AAPS J. 2024) and The MIQE Guidelines for qPCR and dPCR.
Flexible, client-centered solutions
We work closely with our clients to find tailored solutions, offering flexibility in sample types, test items, sample volume, and scalability. We prioritize transparency and proactive communication throughout each project, ensuring our clients are continuously updated on the progress.
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Let's explore how we can support your drug development program.
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