Next Generation Sequencing
TCR/BCR-Seq
Study the diversity of T-cell and B-cell populations, revealing how specific clones expand or contract in response to therapies or diseases at the RNA or DNA level.
T-cell receptor and B-cell receptor sequencing
Using next-generation sequencing, TCR/BCR sequencing offers a highly sensitive assay for studying the immune receptor repertoire (from DNA) and gene expression (from RNA).
Study the diversity of T-cell and B-cell populations, revealing how specific clones expand or contract in response to therapies or disease.
Track expanded clones, providing insights into the specificity and strength of the immune response.
Monitor how T-cell and B-cell repertoires change after therapeutic intervention or vaccination, helping to assess the efficacy and durability of immune memory.
PCR ASSAYS AT TATAA
TATAA Biocenter specializes in developing and validating quantitative nucleic acid analysis (qPCR and dPCR) for bioanalysis, adhering to regulatory requirements for different phases. Our services encompass fit-for-purpose assays to full Good Laboratory Practice (GLP) and Good Clinical Laboratory Practice (GCLP) compliant assays. We ensure GLP accreditation for preclinical and GCLP compliance for clinical samples, meeting FDA and EMA standards.
Our core expertise is quantifying low-copy RNA and DNA in intricate biological samples. Our services encompass consultation on sampling, storage, transportation, experimental planning, instrument selection, and reagent recommendations. We excel in extractions from diverse tissue types, biofluids, and various sampling tools.
TCR/BCR-Seq
Library preparation
Cellecta DriverMap AIR TCR-BCR Profiling kit
CDR3 only or full-length variable region
Recommended sample input
- Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized)
- Tissue or cells (fresh-frozen or stabilized)
- FFPE samples
Recommended DNA input
Range: 1 ng – 1 ug RNA or 0.5-5 ug DNA
Standard input: 100 ng RNA or 2 ug DNA
Recommended sequencing
Paired-end sequencing PE300
Deliverables
Quality-controlled data
Fastq files
Study report
Additional options
Data analysis using MiXCR to for clonotype diversity, overlap, heatmaps and time series
About us
The power of our approach
Committed to quality, innovation, and fostering strong client relationships, we serve as your trusted partner to accelerate drug development. As a contract research organization, our mission is to push the boundaries of science and technology to generate accurate and reproducible data that shortens time-to-market.
Regulated laboratory environment
We are GLP accredited for qPCR, dPCR, and molecular biology, GCLP compliant, and ISO/IEC 17025 accredited. Our facility features a strict sample management process, a fully integrated LIMS system, backup for all vital systems, temperature and humidity control, and robust IT security.
Purpose-built PCR laboratory
Our purpose-built laboratory in Gothenburg, Sweden, is specifically designed for PCR. It has controlled air pressure, temporal separation, and biosafety cabinets to minimize contamination risk. This setup enables us to achieve the highest sensitivity and robustness required for validated assays. The lab is equipped with market-leading PCR and NGS instruments and advanced liquid handling systems.
Pioneers in assay development and validation
We are a team of 45 employees, with scientists at the forefront of assay development, optimization, and validation. Our team has co-authored key publications in the field, including Recommendations for Method Development and Validation of qPCR and dPCR Assays in Support of Cell and Gene Therapy Drug Development (AAPS J. 2024) and The MIQE Guidelines for qPCR and dPCR.
Flexible, client-centered solutions
We work closely with our clients to find tailored solutions, offering flexibility in sample types, test items, sample volume, and scalability. We prioritize transparency and proactive communication throughout each project, ensuring our clients are continuously updated on the progress.
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Let's explore how we can support your drug development program.
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