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BCR sequencing service

B-cell receptor sequencing services for analyzing the B-cell repertoire and clonal diversity in advanced cell and gene therapies.

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BCR sequencing services at TATAA Biocenter

We offer BCR sequencing services for B-cell receptor sequencing, analyzing the full-length B-cell receptor repertoire or targeting only the CDR3 repertoire across IGH, IGK, and IGL chains. Our BCR repertoire sequencing workflow is based on RT-PCR and next-generation sequencing (NGS), running in a single reaction to ensure efficient and reproducible results. These assays support human and mouse samples, parallel processing RNA, DNA, or both for a complete BCR profiling approach.

B-cell receptor transcripts (RNA): Facilitates high-sensitivity detection of rare BCR clonotypes, enabling in-depth BCR repertoire analysis of active B-cell responses and antibody production.

B-cell receptor genes (DNA): Provides quantitative assessment of genetic copies of each CDR3-specific clonotype, reflecting the number of B cells carrying that clonotype in the sample. This is particularly useful for studying B-cell clonal expansion, BCR repertoire diversity, and immune memory development.

TCR and BCR sequencing in a single reaction

T-cell receptor (TCR) and B-cell receptor (BCR) sequencing allow for comprehensive TCR/BCR repertoire analysis, providing deeper insights into immune function, clonal diversity, and antigen-specific responses. By parallel leveraging BCR sequencing and TCR sequencing, researchers can study B-cell receptor repertoire diversity and TCR repertoire profiling, offering a complete picture of adaptive immunity.

Our workflows enable BCR repertoire sequencing and TCR repertoire sequencing to be performed simultaneously using a single RNA and DNA kit. This approach ensures efficient, high-resolution BCR profiling and TCR clonotype analysis, supporting vaccine development, immunotherapy, and disease monitoring applications.

We provide robust solutions to analyze TCR and BCR repertoires for a complete immune repertoire analysis, offering deep insights into immune system function across various diseases and therapeutic interventions.

GLP and GCLP compliant laboratory

We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.

BCR sequencing analysis with MiXCR

We offer B-cell receptor (BCR) sequencing services to study the adaptive immune system, providing insights into BCR repertoire diversity, clonal expansion, antigen-specific responses, and somatic hypermutation patterns. Our high-throughput sequencing workflows enable accurate characterization of B-cell dynamics in various research and clinical applications.

As part of our BCR sequencing analysis pipeline, we use MiXCR, a widely recognized bioinformatics tool for processing raw sequencing data and extracting detailed insights into:

B-cell clonal expansion and lineage tracking
BCR repertoire diversity and immune profiling
Antigen-specific B-cell responses
Somatic hypermutation and affinity maturation

MiXCR aligns sequencing reads to BCR reference databases (e.g., IMGT) to identify IGH, IGK, and IGL gene segments, ensuring precise BCR repertoire sequencing. The final analysis generates comprehensive reports on clonal diversity, clonal abundance, and immune response profiling, enabling deeper insights into vaccine response, immunotherapy, autoimmune diseases, and oncology research.