Illumina Protein Prep (IPP)
One of the few service centers worldwide equipped with the modified Tecan robot for the new fully automated Illumina Protein Prep (IPP) workflow, providing Illumina proteomic services.
The world-leading next-generation sequencing provider introduces an innovative protein quantification solution using SOMAmer™ technology and Illumina NGS readouts.
- > 9000 unique human protein targets
- 200+ biological pathways
- 10 log dynamic range between lowest and highest protein abundance
- Median CV of ~ 6 %
- Fully automated workflow
- Overlay with transcriptomics data for a multiomic approach
Maximize discovery potential with Illumina proteomics
The Illumina Protein Prep (IPP) solution leverages the renowned sensitivity, precision, and scalability of next-generation sequencing (NGS) technologies, extending these capabilities to proteomics. This scalable, automated workflow is designed to bring the powerful exploratory potential of genomics and transcriptomics to protein analysis.
The readout is performed on the NovaSeq 6000, one of Illumina’s most advanced high-throughput sequencing platforms, delivering massive, high-speed sequencing capacity.
Contact us for a complete protein list.
The IPP workflow:
Capture
Target proteins are captured on magnetic beads using SOMAmer™ reagents.
Biotinylation
The proteins bound to the beads are then biotinylated.
Photo-cleavage
UV light breaks the linker, releasing the now biotinylated proteins bound to SOMAmer™ reagents from the beads.
Streptavidin capture
The released proteins are captured using streptavidin beads, which bind to the biotin.
Next-generation sequencing
The SOMAmers are eluted, barcoded, and sequenced. The quantification of SOMAmers serves as a proxy for the protein concentration in the sample.
GLP and GCLP compliant laboratory
We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.
Resources
Overview of bioanalysis in advanced therapies
- Assay design and validation
- Regulatory guidelines (EMA and FDA)
- Challenges and best practices
Genomic biomarker analysis (DNA, RNA, miRNA, ctDNA etc,)
- Technology overview
- Analytical validation
- Regulatory overview
Capability PDF
Download our latest brochure for a summary of our molecular CRO services and capabilities.