Technologies → RNA-Seq

RNA sequencing services

Bulk RNA-Seq analysis and RNA-Seq services supporting discovery, preclinical, and clinical applications.

GCLP-compliant RNA sequencing for clinical applications

Optimized, automated workflows for RNA-Seq analysis services across all phases of drug development.

Our transcriptomic next-generation sequencing (NGS) bulk RNA-Seq support projects of all sizes, from small to large cohorts, in regulated (GLP/GCLP) and non-regulated studies.

RNA-Seq technologies for transcriptome analysis

Total RNA-Seq

mRNA-Seq

3’-mRNA-Seq

Small RNA-Seq

TCR-Seq

BCR-Seq

GLP and GCLP compliant laboratory

We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.

RNA-Seq at TATAA

Samples

Tissues
Blood
Liquid biopsies
Fresh frozen
FFPE
EDTA
PAXgene tubes

Analytes

Total RNA
mRNA
small RNA
mRNA poly(A) sequences
TCR
BCR

Illumina RNA-seq solutions

Illumina is a leading provider of next-generation sequencing (NGS) technology, offering genomic, transcriptomic, and epigenetic analysis platforms. TATAA Biocenter has several sequencing instruments ranging from low-throughput systems like iSeq to high-throughput platforms like the NovaSeq 6000, supporting applications from targeted sequencing to whole-genome analysis. In addition, Illumina provides a diverse range of library preparation kits optimized for different sequencing needs, ensuring high-quality, reproducible data.

The Illumina Stranded mRNA Prep kit offers a fast, efficient, and highly reproducible workflow for mRNA sequencing. Poly (A) enrichment selectively captures mRNA transcripts, effectively removing ribosomal RNA (rRNA). This kit is designed for low-input samples, requiring as little as 25 ng of total RNA, and is scalable across Illumina platforms, from MiSeq to NovaSeq.

The Illumina Total RNA Prep kit is designed for whole transcriptome analysis. It preserves a broad range of RNA species, including non-polyadenylated RNAs such as long non-coding RNAs (lncRNA) and small RNAs. Unlike mRNA prep kits, it employs rRNA depletion instead of poly(A) selection, making it ideal for comprehensive transcriptome profiling. This kit is compatible with low-input samples, starting from just 1 ng of total RNA.

Our custom RNA-Seq services are tailored to each project’s needs. We select the optimal platform and library preparation kit based on sample type, study objectives, and regulatory requirements.

FFPE RNA-Seq

Formalin-fixed, paraffin-embedded (FFPE) samples often contain degraded and crosslinked RNA due to the fixation process. To address this, we use a 3′ mRNA sequencing library preparation kit optimized for gene expression analysis in FFPE samples. This approach selectively sequences the 3′ untranslated region (UTR) of mRNA, making it highly suitable for low-quality and fragmented RNA derived from FFPE tissues.

RNA-Seq on blood, plasma and tissues

RNA-Seq, including both extraction and library preparation, varies depending on the RNA source, RNA composition, and quality. FFPE-fixed tissues often yield degraded RNA, requiring specialized protocols, while fresh tissues provide high RNA yield and typically require rRNA depletion. Whole blood contains high levels of globin mRNA, necessitating globin depletion for accurate transcriptome profiling. Low RNA input samples, such as cfRNA from plasma, require ultrasensitive library preparation kits to capture fragmented and low-abundance transcripts.

Upon sampling, it is crucial to stabilize RNA, inhibit nuclease activity, and minimize RNA degradation. PAXgene blood collection tubes contain a specialized reagent that lyses cells upon collection, preventing RNA degradation. We have developed optimized, automated workflows for RNA extraction from PAXgene tubes, demonstrating consistently high RNA Quality Number (RQN) and RNA Integrity Number (RIN) values.

Specialized blood collection tubes are required to collect plasma and cell-free RNA (cfRNA) and prevent cell lysis and RNA degradation.

RNA in fresh biopsy tissues is highly susceptible to degradation by RNases. To minimize degradation, tissues can be flash-frozen in liquid nitrogen or collected in an RNA-stabilizing reagent, such as RNAlater.