Technologies → RNA-Seq → Small RNA-Seq

Small RNA-Seq and miRNA-Seq services for drug development

High-throughput, automated workflows for small RNA sequencing, including microRNAs (miRNAs), small interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs)

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Small RNA-Seq services at TATAA Biocenter

Small RNA-Seq enriches small RNA molecules, such as miRNA and siRNA, providing quantitative insights into how they regulate gene expression at the post-transcriptional level. This method can be used to evaluate the potential of small RNAs as therapeutic targets or to assess the efficacy of drugs that modulate gene expression.

Identify miRNAs and siRNAs involved in disease pathways.
Study RNA interference (RNAi) mechanisms when developing RNA-targeted therapies.
miRNA biomarker discovery and validation in early drug development stages.

Small RNA-Seq enriches for small RNA molecules, such as miRNA, siRNA, and piwi-interacting RNAs (piRNAs).

We perform qPCR and dPCR on short RNA and DNA targets

	Total RNA-Seq	mRNA-Seq	3’-mRNA-Seq	Small RNA-Seq
Library preparation	Total RNA-Seq with UMIs and UDIs, including rRNA depletion, and globin depletion for whole blood samples.	mRNA-Seq with UMIs and UDIs, including polyA selection	3’-mRNA-Seq with UMIs and UDIs	Takara
Recommended sample input	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) Plasma, serum or other biofluids
Recommended RNA input	Range: 1 ng – 1 ug Standard input: 100ng	Range: 1 ng – 1 ug Standard input: 100ng	Range: 1 ng – 1 ug Standard input: 10ng	Range: 0.1 ng – 1 ug Standard input: 100ng
Recommended sequencing	Paired-end sequencing PE100, 35M read-pairs per sample or 50M for blood samples	Paired-end sequencing PE100, 25M read-pairs per sample or 40M for blood samples	Single-read sequencing SR50, 5M read-pairs per sample or 10M for blood samples	Single-read sequencing
Deliverables	Quality-controlled data Fastq files Study report	Quality-controlled data Fastq files Study report	Quality-controlled data Fastq files Study report	Differential gene expression analysis, pathway analysis, custom data analysis (please enquire to discuss further).
Additional options	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis

RNA-seq services

GLP and GCLP compliant laboratory

We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.