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Whole transcriptome sequencing service

GCLP-compliant Total RNA-Seq services for drug development, supporting projects from small sample sets to large clinical cohort studies.

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Comprehensive whole transcriptome analysis at TATAA

Total RNA-Seq captures both coding (mRNA) and non-coding RNAs, including transfer RNA (tRNA), microRNA (miRNA), and long non-coding RNA (lncRNA). If rRNA is not of interest, it is typically removed, as it can dominate sequencing reads and constitute up to 80–90% of total RNA.

Discover novel RNA species and biomarkers
Identify non-coding RNA roles in disease mechanisms
Comprehensive transcriptome profiling for target identification

Total RNA-Seq captures both coding (mRNA) and non-coding RNAs

	Total RNA-Seq	mRNA-Seq	3’-mRNA-Seq	Small RNA-Seq
Library preparation	Total RNA-Seq with UMIs and UDIs, including rRNA depletion, and globin depletion for whole blood samples.	mRNA-Seq with UMIs and UDIs, including polyA selection	3’-mRNA-Seq with UMIs and UDIs	Takara
Recommended sample input	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) Plasma, serum or other biofluids
Recommended RNA input	Range: 1 ng – 1 ug Standard input: 100ng	Range: 1 ng – 1 ug Standard input: 100ng	Range: 1 ng – 1 ug Standard input: 10ng	Range: 0.1 ng – 1 ug Standard input: 100ng
Recommended sequencing	Paired-end sequencing PE100, 35M read-pairs per sample or 50M for blood samples	Paired-end sequencing PE100, 25M read-pairs per sample or 40M for blood samples	Single-read sequencing SR50, 5M read-pairs per sample or 10M for blood samples	Single-read sequencing
Deliverables	Quality-controlled data Fastq files Study report	Quality-controlled data Fastq files Study report	Quality-controlled data Fastq files Study report	Differential gene expression analysis, pathway analysis, custom data analysis (please enquire to discuss further).
Additional options	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis

RNA-seq services

GLP and GCLP compliant laboratory

We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.