Technologies → RNA-Seq → 3’-mRNA-Seq

3’ mRNA-Seq services

Optimized and automated 3′ mRNA-Seq services for highly efficient, large-scale, and reproducible gene expression analysis.

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3’ mRNA-seq services at TATAA Biocenter

3′-mRNA-seq only sequences the 3′ end of mRNA, simplifying library preparation and reducing the amount of sequencing required, making it a cost-effective option. It serves as a faster and more affordable alternative to full-length RNA sequencing when the goal is to measure transcript abundance rather than discover novel transcripts or splice variants.

High-throughput, cost-effective gene expression profiling in large-scale drug screens
Differentially expressed genes in degraded samples, such as FFPE
Biomarker identification and target validation in high-throughput applications

3′-mRNA-seq sequences only the 3′-end of transcripts as a marker for the entire transcript, minimizing the amount of sequencing required.

	Total RNA-Seq	mRNA-Seq	3’-mRNA-Seq	Small RNA-Seq
Library preparation	Total RNA-Seq with UMIs and UDIs, including rRNA depletion, and globin depletion for whole blood samples.	mRNA-Seq with UMIs and UDIs, including polyA selection	3’-mRNA-Seq with UMIs and UDIs	Takara
Recommended sample input	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) FFPE sections from resected tissue or biopsies	Whole blood (e.g. PAXgene Blood RNA Tubes) or PBMCs (fresh-frozen or stabilized) Tissue or cells (fresh-frozen or stabilized) Plasma, serum or other biofluids
Recommended RNA input	Range: 1 ng – 1 ug Standard input: 100ng	Range: 1 ng – 1 ug Standard input: 100ng	Range: 1 ng – 1 ug Standard input: 10ng	Range: 0.1 ng – 1 ug Standard input: 100ng
Recommended sequencing	Paired-end sequencing PE100, 35M read-pairs per sample or 50M for blood samples	Paired-end sequencing PE100, 25M read-pairs per sample or 40M for blood samples	Single-read sequencing SR50, 5M read-pairs per sample or 10M for blood samples	Single-read sequencing
Deliverables	Quality-controlled data Fastq files Study report	Quality-controlled data Fastq files Study report	Quality-controlled data Fastq files Study report	Differential gene expression analysis, pathway analysis, custom data analysis (please enquire to discuss further).
Additional options	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis	Differential gene expression analysis, pathway analysis, cell subset deconvolution, custom data analysis

RNA-seq services

GLP and GCLP compliant laboratory

We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.