Whole exome sequencing service
Optimized and automated workflows for next-generation sequencing (NGS) of the full exome in drug development.
WES services at TATAA
Whole Exome Sequencing (WES) is often used in medical research and diagnostics to identify genetic mutations associated with diseases. The coding regions make up only 1–2% of the genome but harbor approximately 85% of known disease-related variants.
- Similar applications to WGS, but focuses on exonic regions only.
- Does not capture structural variants, non-coding regions, regulatory elements, or copy number variants.
- More cost-effective and data-efficient compared to WGS.
Whole exome sequencing (WES) focuses exclusively on coding exons, excluding non-coding introns.
| Whole genome sequencing | Whole exome sequencing | Targeted Gene Panels | Circulating Tumor DNA (ctDNA) | |
| Library preparation | IDT xGen DNA Library Prep Kit EZ with UDIs | IDT xGen DNA Library Prep Kit EZ with UDIs, combined with xGen Exome Hyb Panel v2 | ||
| Recommended sample input | Fresh frozen cells or tissue EDTA whole blood or PAXgene DNA tubes High quality FFPE (IDT DNA Integrity score > 1.0) |
Fresh frozen cells or tissue EDTA whole blood or PAXgene DNA tubes High quality FFPE (IDT DNA Integrity score > 1.0) |
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| Recommended DNA input | Range: 0.1 ng – 1 ug Standard input: 100ng |
Range: 0.1 ng – 1 ug Standard input: 100ng |
Range: 0.1 ng – 1 ug Standard input: 100ng |
Range: 0.1 ng – 1 ug Standard input: 100ng |
| Recommended sequencing | Paired-end sequencing | Paired-end sequencing | Paired-end sequencing | Paired-end sequencing |
| Deliverables | Quality-controlled data Fastq files Study report |
Quality-controlled data Fastq files Study report |
Quality-controlled data Fastq files Study report |
Quality-controlled data Fastq files Study report |
GLP and GCLP compliant laboratory
We are accredited for Good Laboratory Practice (GLP) by the Swedish Board for Accreditation and Conformity Assessment (SWEDAC) for qPCR, dPCR, and molecular biology. In addition, we are Good Clinical Laboratory Practice (GCLP) compliant to ensure the safe and reliable analysis of clinical samples.
Resources
Overview of bioanalysis in advanced therapies
- Assay design and validation
- Regulatory guidelines (EMA and FDA)
- Challenges and best practices
Genomic biomarker analysis (DNA, RNA, miRNA, ctDNA etc,)
- Technology overview
- Analytical validation
- Regulatory overview
Capability PDF
Download our latest brochure for a summary of our molecular CRO services and capabilities.